Introduction to Enzyme Kinetics

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What is enzyme kinetics?

Enzyme kinetics is the study of the rates at which enzyme-catalyzed reactions proceed and the factors affecting these rates.

What is the Michaelis-Menten equation?

The Michaelis-Menten equation describes the rate of enzyme-catalyzed reactions and is given by v = (Vmax[S])/(Km + [S]).

What does the term 'Vmax' signify in enzyme kinetics?

Vmax represents the maximum rate of an enzyme-catalyzed reaction, achieved when the enzyme is saturated with substrate.

Define Km in the context of enzyme kinetics.

Km is the Michaelis constant, representing the substrate concentration at which the reaction rate is half of Vmax.

What is the Lineweaver-Burk plot?

The Lineweaver-Burk plot is a double-reciprocal graph used to determine Km and Vmax from enzyme kinetics data.

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