Introduction to Tissue Culture

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What is tissue culture?

Tissue culture is a technique of growing cells, tissues, or organs in a nutrient medium under sterile conditions outside an organism.

What are the main applications of tissue culture?

Tissue culture is mainly used for plant propagation, genetic modification, research, and producing virus-free plants.

Why is sterility crucial in tissue culture?

Sterility is crucial because contamination by microorganisms can interfere with the growth of cells and result in the failure of the culture.

What is the role of the nutrient medium in tissue culture?

The nutrient medium supplies essential nutrients, vitamins, and growth hormones necessary for cell growth and development.

What are explants in tissue culture?

Explants are pieces of plant or animal tissue taken for use in tissue culture, from which new cells will grow.

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