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What is micropropagation?
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Micropropagation is a method of rapidly multiplying stock plant material to produce many progeny plants using modern plant tissue culture methods.
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What are the main stages of micropropagation?
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The main stages are: 1) Establishment, 2) Multiplication, 3) Rooting or pre-plant stage, 4) Acclimatization.
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Why is sterile technique important in micropropagation?
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Sterile technique is crucial in micropropagation to prevent contamination and ensure the growth of healthy plant tissues.
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How does micropropagation benefit agriculture?
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Micropropagation allows for the rapid production of disease-free, high-quality, and genetically identical crops, enhancing agricultural productivity.
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What is the role of plant growth regulators in micropropagation?
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Plant growth regulators control cell division and elongation, differentiation, and development of plant tissues in micropropagation.
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What types of cultures are used in micropropagation?
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Types of cultures include callus culture, cell suspension culture, meristem culture, and organ culture.
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What is the 'conditioning' step in micropropagation?
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Conditioning involves gradually adapting plants to outdoor conditions post-culture, often using a greenhouse environment.
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Why is micropropagation used for endangered species?
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Micropropagation is used to conserve endangered species by producing large quantities of plants for reintroduction without extracting from already stressed wild populations.
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What are the limitations of micropropagation?
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Limitations include high initial costs, the necessity for technical expertise, and potential for somaclonal variation in plants.
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What is somaclonal variation?
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Somaclonal variation refers to genetic variation observed among progeny plants produced through plant tissue culture.
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How do micropropagated plants differ from traditionally propagated plants?
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Micropropagated plants are genetically identical to the parent plant and are grown in lab-controlled environments, unlike traditionally propagated plants which can vary genetically.
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What is meristem culture and why is it important?
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Meristem culture involves culturing the plant's meristematic tissue and is significant for producing virus-free plants.
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What types of plants are commonly micropropagated?
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Plants that are commonly micropropagated include orchids, bananas, strawberries, and ornamental plants.
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How do culture media components affect micropropagation?
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Culture media components such as nutrients, vitamins, and growth regulators crucially influence cell growth and differentiation.
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What environmental factors are controlled during micropropagation?
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Key environmental factors include light intensity, temperature, humidity, and atmospheric composition.
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